Heterologous expression of functional G‐protein‐coupled receptors in Caenorhabditis elegans
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چکیده
منابع مشابه
Heterologous expression of functional G-protein-coupled receptors in Caenorhabditis elegans.
New strategies for expression, purification, functional characterization, and structural determination of membrane-spanning G-protein-coupled receptors (GPCRs) are constantly being developed because of their importance to human health. Here, we report a Caenorhabditis elegans heterologous expression system able to produce milligram amounts of functional native and engineered GPCRs. Both bovine ...
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1 Grupo de Genômica e Biologia Computacional, Centro de Pesquisas René Rachou, Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais, Fundação Oswaldo Cruz FIOCRUZ, Belo Horizonte, Brazil 2 Genetics Department, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil 3 Núcleo de Pesquisa em Ciências Biológicas, Universidade Federal de Ouro Preto, ...
متن کاملExpression of mammalian G protein-coupled receptors in Caenorhabditis elegans.
Constituting the largest group of membrane proteins identified in the human genome, G protein-coupled receptors (GPCRs) help control many physiological processes by responding to various stimuli. As targets for more than 40% of all prescribed pharmaceuticals, detailed understanding of GPCR structures is vital for the design and development of more specific medications and improved patient thera...
متن کاملTocotrienol Modulates the Expression of Proteins in Oxidative Stress-Induced Caenorhabditis Elegans
Objective: Oxidative stress that damages proteins result in aging and age related diseases. The aim of this study is to determine the effect of tocotrienol rich fraction (TRF) on the expression of proteins in oxidative stress-induced caenohabditis elegans (C.elegans) which has homologous genes to humans. Methods: The worms were treated with TRF prior to, after and continuously in separate group...
متن کاملTocotrienol Modulates the Expression of Proteins in Oxidative Stress-Induced Caenorhabditis Elegans
Objective: Oxidative stress that damages proteins result in aging and age related diseases. The aim of this study is to determine the effect of tocotrienol rich fraction (TRF) on the expression of proteins in oxidative stress-induced caenohabditis elegans (C.elegans) which has homologous genes to humans. Methods: The worms were treated with TRF prior to, after and continuously in separate group...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2011
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fj.11-197780